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E coli in gram stain E coli in Gram stain showing gram negative rods having size of about µm long and diameter as shown above picture Introduction of Escherichia coli E coli is a Gram negative, aerobe and facultative anaerobic, rodshaped bacteria Optimal temperature for growth is 3617°C with most strains growing over the range 1844 °CA slide is also not acceptable for examination if microorganisms that should be grampositive appear pink This may indicate that either the Gram's iodine was not applied or the slide was overdecolorized Staining grampositive and gramnegative control slides along with the patient's smear would confirm that proper staining technique was usedOn Endo agar it looks like lactose negative)All four strains are mannitol positive (best seen in fig D), cellobiose negative (strains A, B) Gram Staining Principle Procedure And Results Learn Microbiology Online E coli gram stain slide